Comparison of Al-induced gene expression in sensitive and tolerant soybean cultivars
2003
Ermolayev, V. | Weschke, W. | Manteuffel, R.
In order to identify genes involved in soybean resistance to aluminium (Al) stress differential gene expression patterns of Al-stressed and non-stressed tolerant and sensitive soybean cultivars were compared. Out of eight described genes, potentially related to mechanisms of aluminium stress, only phosphoenolpyruvate carboxylase (PEPC) revealed enhanced expression in roots of tolerant as compared to sensitive soybean cultivars under stress conditions. Additionally, two novel full-length cDNA sequences, homologous to translationally controlled tumour proteins (TCTP, clone 58, GenBank accession number AF421558) and inosine-5'-monophosphate dehydrogenases (IMPDH, clone 633, GenBank accession number AF421559) with enhanced expression of the corresponding genes only in roots of Al-tolerant soybean cultivar under stress conditions were isolated and characterized. For functional analysis full-length cDNA 633 was transferred in Arabidopsis thaliana. Only 6% of the seedlings from the wild type survived Al stress, whereas 86% of transgenics were vital demonstrating superiority in stress protection. Compared with the wild type, transgenic plants showed diminished Al penetration into the roots after the stress treatment especially in the division and elongation zones of the roots. Formation of numerous lateral roots in transgenic plants with low elicited callose accumulation under stress conditions indicated ability of the IMPDH homologue to mediate aluminium tolerance in transgenic plants. Possible functional activities of Al up-regulated genes in resistance mechanisms are discussed.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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