Pfaffia paniculata (Brazilian ginseng) extract modulates Mapk and mucin pathways in intestinal inflammation
2017
Costa, C.A.R.A. | Quaglio, A.E.V. | Di Stasi, L.C.
Pfaffia paniculata is endemic Brazilian plant traditionally used against fatigue, stress, inflammation and low immune system functioning and with proven intestinal anti-inflammatory activity.To evaluate intestinal anti-inflammatory mechanism of P. paniculata by determination of mRNA abundance of Hsp70, Heparanase, Mapk1, Mapk3, Mapk6, Mapk9, Muc1, Muc2, Muc3, Muc4, and NF-κB, as well as the mucin content in colonic samples.Intestinal inflammation was induced by TNBS and rats were divided into groups that received vehicle or 25, 50, 100, or 200mg/kg of P. paniculata extract, p.o., started 2h after inflammation induction and continued daily for 7 days. At the end of the procedure, the animals were killed and their colon samples were obtained for RT-qPCR analysis and mucin histochemical study with PAS/Alcian blue stain.P. paniculata significantly decreased Hsp70 (25mg/Kg); reduced Mapk3 and increased Muc4 (50mg/Kg); increased Mapk, Muc3, Muc4 with simultaneous decrease in Mapk3 (100mg/kg) and, reduced Mapk3at 200mg/kg. At 50–100mg/Kg, P. paniculata increased mucins secretions.Intestinal anti-inflammatory activity of P. paniculata was related to modulation of the Mapks and mucin gene expression and mucus secretion in intestinal inflammation.
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