Growth Enhancement of Chickpea in Saline Soils Using Plant Growth-Promoting Rhizobacteria
2012
Patel, Dhara | Jha, Chaitanya Kumar | Tank, Neelam | Saraf, Meenu
Bacterial isolates with the ability to tolerate salinity and plant growth-promoting features were isolated from the saline areas of Gujarat, India, that is, Bhavnagar and Khambat. A total of 176 strains of rhizobacteria were isolated out of which 62 bacterial strains were able to tolerate 1Â M NaCl. These were then further studied for their potential plant growth-promoting rhizobacteria characteristics like phosphate solubilization, siderophore production, and IAA production. Twenty-eight isolates of the 62 strains showed good tricalcium phosphate solubilization in solid medium in the range of 9–22Â mm and 15 isolates showed good phosphate solubilization in liquid medium in the range of 9–45Â μg/ml. Siderophore production was checked in all 15 isolates, and 13 were screened out that produced the hydroxamate type of siderophore in the range of 11–50Â mM. Among the 13 isolates, 10 were able to produce indole acetic acid in the range of 10–26Â μg/ml after 72Â h of incubation. Pot trials were carried out on chickpea under 300Â mM NaCl stress using the best five isolates. Plants inoculated with MSC1 or MSC4 isolates showed an increase in the parameters that evaluate plant growth when compared to uninoculated controls. Strains MSC1 and MSC4 were identified as Pseudomonas putida and Pseudomonas pseudoalcaligens, respectively, according to sequence analysis of the 16S rRNA gene.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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