Profenofos insecticide degradation by novel microbial consortium and isolates enriched from contaminated chili farm soil

Siripattanakul-Ratpukdi, Sumana; Vangnai, Alisa S.; Sangthean, Puttaporn; Singkibut, Sirinuttakan

Profenofos insecticide degradation by novel microbial consortium and isolates enriched from contaminated chili farm soil

2015

Siripattanakul-Ratpukdi, Sumana | Vangnai, Alisa S. | Sangthean, Puttaporn | Singkibut, Sirinuttakan

Profenofos (PF) is one of the heavily used organophosphorus pesticides (OPPs) of which its contamination is ubiquitous in an agricultural area. This study aims to acquire and characterize PF-degrading bacterial cultures from contaminated soil. OPP degradation by the novel isolates was then investigated. The experiment was performed at the initial PF concentration of 20 mg/L. The result showed that the enriched consortium comprised three predominant PF-degrading strains designated as PF1, PF2, and PF3. The isolates (PF1, PF2, and PF3) were characterized as Pseudomonas plecoglossicida, Pseudomonas aeruginosa, and P. aeruginosa, respectively. A consortium and all isolates could utilize PF as a sole carbon source with PF removal of more than 90 % via a hydrolysis process. The bacterial growth and PF degradation rates followed the first-order kinetic reaction with the rates of 0.4 to 2.7/h and 0.15 to 1.96/h, respectively. Additional carbon supplement deteriorated PF biodegradation. The enriched cultures were also capable for degrading chlorpyrifos and dicrotophos pesticides (33–73 % removal). The results indicated that the consortium and isolates are efficient for PF and other OPP degradation and have potential for PF remediation.

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