Complementarity of bioassays and microbial activity measurements for the evaluation of hydrocarbon-contaminated soils quality
2001
Brohon, B. | Delolme, C. | Gourdon, R.
In order to describe a soil polluted with hydrocarbons, the complementarity of bioassays and microbial activities measurements was studied. The samples of soil were taken from a site which had received oil tank residues over 50 years. Five zones were sampled. Each sample was characterized by chemical analyses, the measurement of dehydrogenase, phosphatase, hydrolysis of FDA and urease activities, soil respiration, and Microtox and Metplate bioassays. The chemical analyses revealed different levels of total hydrocarbon concentrations (from 1.5 to 78.8 mg/kg of dry soil) but also relatively high quantities of nickel (from 14.5 to 841.6 mg/kg of dry soil) and lead (30.9-355.4 mg/kg of dry soil) or cadmium (0-1.2 mg/kg of dry soil) in the different zones. Urease and dehydrogenase were sensitive to the presence of metals (31% inhibition of urease and 50% inhibition of dehydrogenase in the most contaminated soil). Measurements of Substrate Induced Respiration showed that the soil microflora were stressed in the presence of the pollutants. In the zone containing the highest concentration of metals, the microbial activities were low and the bioassays revealed a high potential toxicity (e.g. IC50 for Microtox obtained with a 15% dilution of soil, 90% inhibition of beta-galactosidase activity). In the other zones, the soil microbial activities were not depressed in comparison to the reference zone whereas the bioassays revealed the presence of toxic compounds extracted with the solvent used.
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