Characterization of chromosomal rearrangements in pileated gibbon (Hylobates pileatus) using multiplex-FISH technique
2016
Sangpakdee, Wiwat | Phimphan, S. | Liehr, T. | Fan, X. | Pinthong, K. | Patawang, I. | Tanomtong, A.
Here we aimed to characterize evolutionary conserved chromosomal rearrangements of pileated gibbon (H. pileatus, HPI) compared to human using multiplex fluorescence in situ hybridization (M-FISH) technique. Khon Kaen Zoo, Thailand, kindly provided the blood specimen of this gibbon. Lymphoblast cells were cultured from male HPI by standard method; chromosome preparation was done following standard protocols. All 24 human (Homo sapiens, HSA) chromosome probes (M-FISH probe set) were applied on HPI. Results indicated that male HPI had diploid chromosome numbers of 2n = 44; the karyotype consists of metacentric and submetacentric chromosomes. During evolution chromosomes of HPI underwent certain rearrangements when compared with human chromosomes and showed 55 conserved regions of different sizes. Majority of HPI chromosomes are constituted of regions being homologous to two or more human chromosomes. The remainder appeared to be conserved, such as HPI chromosomes 14, 17, 18, 20, 21, X and Y which were homologous to only one human chromosome, each. To the best of our knowledge, this is the first study using all 24 HSA probes on HPI; the genetic data may be additionally useful for comparison of gibbons, both within and between genera, to elucidate and clarify more regarding evolutionary lineage of animals in the order primates derived from the same common ancestor known so far.
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