Resistance to the cereal cyst nematode (Heterodera avenae Woll.) transferred from the wild grass Aegilops ventricosa to hexaploid wheat by a "stepping stone" procedure

Delibes, A.; Romero, D.; Aguaded, S.; Duce, A.; Mena, M.; Lopez-Brana, I.; Andres, M.F.; Martin Sanchez, J.A.; Garcia-Olmedo, F.

Resistance to the cereal cyst nematode (Heterodera avenae Woll.) transferred from the wild grass Aegilops ventricosa to hexaploid wheat by a "stepping stone" procedure

1993

Transfer of resistance to Heterodera avenae, the cereal cyst nematode (CCN), by a "stepping-stone" procedure from the wild grass Aegilops ventricosa to hexaploid wheat has been demonstrated. The number of nematodes per plant was lower, and reached a plateau much earlier, in the resistant introgression line H93-8 (1-2 nematodes per plant) than in the recipient H10-15 wheat (14-16 nematodes per plant). Necrosis (hypersensitive reaction) near the nematode, little cell fusion, and few, often degraded syncytia were observed in infested H93-8 roots, while abundant, well-formed syncytia were present in the susceptible H10-15 wheat. Line H93-8 was highly resistant to the two Spanish populations tested, as well as the four French races (Fr1-Fr4), and the British pathotype Ha11, but was susceptible to the Swedish pathotypes HgI and HgIII. Resistance was inherited as though determined by a single quasi-dominant factor in the F2 generations resulting from crosses of H93-8 with H10-15 and with Loros, a resistant wheat carrying the gene Cre1 (syn. Cen1). The resistance gene in H93-8 (Cre2 or Ccn2) is not allelic with respect to that in Loros. RFLPs and other markers, together with the cytogenetical evidence, indicate that the Cre2 gene has been integrated into a wheat chromosome without affecting its meiotic pairing ability. Introduction of Cre2 by backcrossing into a commercial wheat background increases grain yield when under challenge by the nematode and is not detrimental in the absence of infestation.

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