Copper Staining Method for Extracting Biologically Active Proteins from Native Gels
2001
Wen, Jie | Zhang, Mei | HORAN, Thomas P. | PHILO, John S. | Li, Tiansheng | WYPYCH, Jette | MENDIAZ, Elizabeth A. | LANGLEY, Keith E. | AOKI, Kenneth H. | KUWAMOTO, Moeka | KITA, Yoshiko | ARAKAWA, Tsutomu
An attempt was made to make protein bands visible on native gel using copper staining, since such a mild staining procedure would make the entire native gel electrophoresis process non-denaturing. Copper staining not only was able to detect various proteins on native gel with reasonable sensitivity, but also made extraction and recovery of active proteins possible from the gel using a gentle procedure.
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