Simultaneous monitoring of superoxides and intracellular calcium ions in neutrophils by chemiluminescence and fluorescence: Evaluation of action mechanisms of bioactive compounds in foods
2013
Kazumura, Kimiko | Sato, Yukiko | Satozono, Hiroshi | Koike, Takashi | Tsuchiya, Hiroshi | Hiramatsu, Mitsuo | Katsumata, Masakazu | Okazaki, Shigetoshi
We have developed a measuring system for simultaneous monitoring of chemiluminescence and fluorescence, which indicate respectively, (i) generation of superoxide anion radicals (O₂ ⁻•) and (ii) change in the intracellular calcium ion concentration ([Ca²⁺]ᵢ) of neutrophils triggered by the mechanism of innate immune response. We applied this measuring system for establishing a method to distinguish between anti-inflammatory actions and antioxidant actions caused by bioactive compounds. We evaluated anti-inflammatory agents (zinc ion [Zn²⁺] and ibuprofen) and antioxidants (superoxide dismutase [SOD] and ascorbic acid). It was shown that ibuprofen and Zn²⁺ were anti-inflammatory while SOD and ascorbic acid were anti-oxidative. We conclude that it is possible to determine the mechanism of action of bioactive compounds using this method.
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