Concentration of ω-3 polyunsaturated fatty acids of marine oils using Candida cylindracea lipase: Optimization of reaction conditions
1998
Wanasundara, Udaya N. | Shahidi, Fereidoon
Production of ω-3 fatty acid concentrates from seal blubber oil (SBO) and menhaden oil (MHO) upon enzymatic hydrolysis by Candida cylindracea lipase was optimized. In this process, the content of total ω-3 fatty acids, Y ₁; eicosapentaenoic acid, Y ₂; and docosahexaenoic acid, Y ₃, in the final product was maximized. A three-factor central composite rotatable design was used to study the effect of enzyme concentration (X ₁), reaction time (X ₂), and reaction temperature (X ₃). Second-order polynomial regression models for Y ₁, Y ₂, and Y ₃ were employed to generate response surfaces. After hydrolysis, a maximum of 54.3% total ω-3 fatty acids was obtained from SBO at an enzyme concentration of 308 U/g oil, a reaction time of 40 h, and a reaction temperature of 37°C. Similarly, a maximum of 54.5% total ω-3 fatty acids was obtained from MHO at an enzyme concentration of 340 U/g oil, a reaction time of 45 h, and a reaction temperature of 38°C.
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