Occurrence of 28- and 27-carbon ecdysteroids and sterols in developing worker pupae of the leaf-cutting ant Acromyrmex octospinosus (Reich) (Hymenoptera, Formicidae: attini)

Maurer, P.; Royer, C.; Mauchamp, B.; Porcheron, P.; Debieu, D.; Riba, G.

Occurrence of 28- and 27-carbon ecdysteroids and sterols in developing worker pupae of the leaf-cutting ant Acromyrmex octospinosus (Reich) (Hymenoptera, Formicidae: attini)

1991

The major ecdysteroids in large worker pupae of the leaf-cutting ant Acromyrmex octospinosus were characterized at the peak ecdysteroid concentration by using high-performance liquid chromatography, enzyme immunoassay, and mass spectrometry. In decreasing amounts, they were determined to be makisterone A, an unidentified C28 ecdysteroid bearing a molecular weight of 494, 20-hydroxyecdysone (ratio of 1 to 6 as compared to makisterone A), and putative but negligible ecdysone. The presence of both C28 and C27 ecdysteroids is discussed in relation to the content of 4-desmethylsterols determined by gas chromatography and mass spectrometry to be ergosta-5,7,24 (28)-trien-3 beta-ol, ergosterol, ergosta-5,7-dien-3 beta-ol and ergosta-7,24(28)-dien-3 beta-ol 30-of for the main sterols, and with a small amount of cholesterol.

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