Characterization of β-glucosidase from corn stover and its application in simultaneous saccharification and fermentation
2008
Han, Yejun | Chen, Hongzhang
Purification and characterization of β-glucosidase from corn stover was performed and the enzyme was tried in SSF to evaluate the suitability of plant glycosyl hydrolases in lignocellulose conversion. A β-glucosidase with M w of 62.4kDa was purified to homogeneity from post-harvest corn stover. The following physicochemical and kinetic parameters of the β-glucosidase were studied respectively: optimum temperature, thermal stability, optimum pH, pH stability, K m, V max, V i, cellobiose inhibition, tryptic peptide mass spectrometry and effect of metal ions and other reagents on the activity. The β-glucosidase activity on salicin was optimal at pH 4.8 and 37°C. The unique property of optimum temperature makes the β-glucosidase potentially useful in SSF. In SSF of steam explosion pretreated corn stover, the supplementation of the purified β-glucosidase was more effective than Aspergillus niger β-glucosidase.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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