Cadmium effect on vacuolar H⁺-ATPase gene expression in the roots of barley seedlings of different age
2013
Kaznina, N. M. | Titov, A. F. | Topchieva, L. V. | Laidinen, G. F. | Batova, Yu. V.
Expression of two genes (HvVHA E and HvVHA c) of vacuolar H⁺-ATPase was studied in the cells of barley (Hordeum vulgare L., cv. Zazerskii 85) roots in seedlings of different age in the presence of cadmium (100 μM). Three-day-old seedlings were kept for four days on cadmium solution, which caused suppression of root growth accompanied by an increase in HvVHA E gene expression. In this case, the content of reduced glutathione (GSH) decreased. When seven-day-old seedlings were placed on cadmium solution and kept there for four days, the content of metal in the root was higher, but its growth was not suppressed and expression of both studied genes markedly increased. The content of GSH also apparently increased, ensuring the maintenance of high enzyme activity. A comparison of gene expression encoding two subunits of vacuolar H⁺-ATPase and cadmium resistance of the seedlings of different age showed that the enzyme participates in the mechanism of the improvement of barley tolerance to this metal.
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