Redirecting the electron flow towards the nitrogenase and bidirectional Hox-hydrogenase by using specific inhibitors results in enhanced H₂ production in the cyanobacterium Anabaena siamensis TISTR 8012
2012
Khetkorn, Wanthanee | Baebprasert, Wipawee | Lindblad, Peter | Incharoensakdi, Aran
The inhibition of competitive metabolic pathways by various inhibitors in order to redirect electron flow towards nitrogenase and bidirectional Hox-hydrogenase was investigated in Anabaena siamensis TISTR 8012. Cells grown in BG11₀ supplemented with KCN, rotenone, DCMU, and DL-glyceraldehyde under light condition for 24h showed enhanced H₂ production. Cells grown in BG11 medium showed only marginal H₂ production and its production was hardly increased by the inhibitors tested. H₂ production with either 20mM KCN or 50μM DCMU in BG11₀ medium was 22μmolH₂mgchla⁻¹h⁻¹, threefold higher than the control. The increased H₂ production caused by inhibitors was consistent with the increase in the respective Hox-hydrogenase activities and nifD transcript levels, as well as the decrease in hupL transcript levels. The results suggested that interruption of metabolic pathways essential for growth could redirect electrons flow towards nitrogenase and bidirectional Hox-hydrogenase resulting in increased H₂ production.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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