Monoclonal antibodies which show restricted binding to four Colletotrichum species: C. lindemuthianum, C. malvarum, C. orbiculare and C. trifolii
1992
Pain, N.A. | O'Connell, R.J. | Bailey, J.A. | Green, J.R.
Monoclonal antibodies (MAbs) were raised to Colletotrichum lindemuthianum race gamma germlings. When screened against eleven other Colletotrirhum species using an ELISA, two of these MAbs, UB20 and UB22, showed restricted cross-reaction with three other species, viz. C. malvarum, C. orbiculare and C. trifolii. It was also observed that UB20 and UB29 bound only to isolates of C. lindemuthianum from bean and not cowpea. These findings suggest that the four Colletotrichum species recognized by the two MAbs are very closely related, confirming previous evidence. The MAbs did not bind to species of Fusarium or Phoma. UB20 and UB22 bound to carbohydrate epitopes on two distinct sets of glycoproteins. The glycoproteins recognized by the two MAbs were present on the surface of different infection structures in varying amounts: UB20 antigens were predominantly located on conidia, whereas UB22 antigens appeared to be most abundant in fibrillar material around germ-tubes. Both MAbs labelled the wall and matrix around intracellular infection hyphae. Potential applications of the MAbs for the identification of the above pathogens are discussed.
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