Potentiometric polyphenol oxidase biosensor for sensitive determination of phenolic micropollutant in environmental samples
2020
Yashas, Shivamurthy Ravindra | Sandeep, Shadakshari | Shivakumar, Ballagere Puttaraju | Swamy, Ningappa Kumara
The present study demonstrates the development of polyphenol oxidase (PPO) biosensor for the detection of catechol using strontium copper oxide (SrCuO₂) and polypyrrole nanotubes (PPyNT) matrix. The SrCuO₂ micro-seeds, a perovskite compound, are synthesized by co-precipitation under pH 8.0. The as-synthesized micro-seeds are characterized by scanning electron microscopy (SEM), field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction spectroscopy (XRD). The proposed sensor is fabricated on pencil graphite (P-Gr) by successive deposition of PPyNT, SrCuO₂, and PPO enzyme. The developed PPO/SrCuO₂/PPyNT/P-Gr sensor is characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) techniques. The PPO/SrCuO₂/PPyNT/P-Gr displayed excellent electrocatalytic activity towards the oxidation and detection of catechol. The as-developed sensor showed sensitive response ascribing to limit of detection (LOD) of 0.15 μM and sensitivity of 15.60 μA μM⁻¹ cm⁻². The fabricated sensor exhibited excellent repeatability and longer shelf life. The proposed biosensor finds its application within the broad linear range of 1–50 μM. Real sample analysis of mineral water, tap water, and domestic wastewater using developed sensor showed acceptable recovery. Hence, the biosensor endeavors its application in environmental monitoring and protection.
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