A single-nucleotide polymorphism in tumor necrosis factor-α (−308 G/A) as a biomarker in chronic pancreatitis
2014
Sri Manjari, K. | Jyothy, A. | Shravan Kumar, P. | Prabhakar, B. | Uma Devi, M. | Ramanna, M. | Nallari, Pratibha | Venkateshwari, A.
Chronic pancreatitis is a gradual, long-term inflammation of the pancreas that results in alteration of its normal structure and function. The study aims to investigate the role of −308 (G/A) polymorphism of TNF-α gene in chronic pancreatitis.A total of 200 subjects were included in this case–control study. A total of 100 in patients admitted in the Gastroenterology Unit of Gandhi Hospital and Osmania General Hospital, Hyderabad were included in the present study. An equal number of healthy control subjects were randomly selected for the study. The genotyping of TNF-α gene was carried out by tetra-primer ARMS PCR followed by gel electrophoresis. The TNF-α levels were assayed by enzyme-linked immunosorbent assay.A significant variation with respect to the genotypic and allelic distribution in the disease group when compared to control subjects [OR=2.001 (1.33–3.005), p<0.0001**] was observed. Subjects homozygous for the A allele had higher TNF-α levels compared to G allele.The present study revealed a significant association of the TNF-α gene promoter polymorphism with chronic pancreatitis. Thus, TNF-α genotype can be considered as one of the biological markers in the etiology of chronic pancreatitis.
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