Mitochondrial content and gene expression profiles in oocyte-derived embryos of cattle selected on the basis of brilliant cresyl blue staining
2013
Fakruzzaman, Md | Bang, Jae-Il | Lee, Kyeong-Lim | Kim, Seong-Su | Ha, A-Na | Ghanem, Nasser | Han, Chang-Hee | Cho, Kyu-Woan | White, Kenneth L. | Kong, Il-Keun
The aim of this study was to investigate the developmental rate, lipid and mitochondrial distribution and gene expression in oocyte-derived embryos selected on the basis of brilliant cresyl blue (BCB) staining. Lipid content and mitochondrial distribution in Day 8 blastocysts were evaluated by fluorescence intensity, while gene expression was analyzed by real-time PCR. The proportion of blastocysts (30.9%) was greater (P<0.05) in BCB+ than in BCB− oocytes (13%) but not different (P>0.05) from the control group (28.2%). Total cell number was also greater in BCB+ (155.1±36.2) than in BCB− (116.6±40.5) and control (127.5±35.7) blastocysts. Furthermore, the apoptotic cell number was less in BCB+ (3.7±4.4) than in BCB− blastocysts (8.7±8.7) but not different from the control group (5.9±3.9). BCB+ embryos contained more mitochondria compared to BCB− embryos (P<0.05). There was no significant difference in intercellular lipid accumulation in embryos from all groups. Interferon-τ (IFNτ), transforming growth factor β1 (TGFB1) and secreted seminal-vesicle Ly-6 protein 1 (SSLP1) gene expression was greater in BCB+ than in BCB− blastocysts. By contrast, Bcl2-associated X protein (BAX) and heterogeneous nuclear ribonucleoprotein A2/B1 (HNRNPA2B1) gene expression was greater in BCB− than in BCB+ and control embryos. In conclusion, oocyte-derived embryos selected on the basis of BCB staining showed differences in developmental rate, quality, mitochondrial content and target gene expression compared to control embryos.
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