Exploration of production of C₁₄ and C₁₅ bacillomycin D homologues with enzymatic hydrolysis from maize straws using fed-batch fermentation by Bacillus subtilis NS-174
2020
Qian, Shiquan | Li, Xuejin | Sun, Lu | Shen, Yuanyuan | Ren, Qingyi | Diao, Enjie | Lu, Zhaoxin
A strain with strong antifungal activity, Bacillus subtilis NS-174, was identified and the antifungal compounds were purified and structurally analyzed by high performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS). The effects of the enzymatic hydrolysis and the fermentation strategies on production of the antifungal compounds were also investigated. The results showed that the antifungal compounds were characterized to be C₁₄ and C₁₅ bacillomycin D homologues (C₁₄–₁₅BDs). When 400 mL of the cellulase hydrolysates from the maize straws residues (CHMSRs) was fed into 1.0 L of the batch fermentation broth at 60 h, an output of C₁₄–₁₅BDs (OC₁₄–₁₅BDs) was obtained as 44.84 mg gₘₐᵢzₑ ₛₜᵣₐwₛ⁻¹. Moreover, the fed-batch fermentation was beneficial for the increase of the transcriptional activities of C₁₄–₁₅BDs synthetase genes by up-regulating expression of comA, sigmaH and spo0A, which caused the elevation of C₁₄–₁₅BDs production. The present work provided an effective solution for lipopeptide production in Bacillus subtilis.
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