Antifungal properties of quinoa (Chenopodium quinoa Willd) alkali treated saponins against Botrytis cinerea
2008
Stuardo, Macarena | San Martín, Ricardo
Quinoa (Chenopodium quinoa Willd) is a Latin American food staple readily available in large quantities in Peru, Bolivia and Ecuador. The outer husk of the grain is removed prior to consumption to reduce its bitter taste. At present, quinoa husks are considered as a by-product with no commercial value, despite its high content of triterpenoid saponins (20-30%). Due to this, the present work was undertaken to test if quinoa saponins have antifungal properties against Botrytis cinerea and if this activity is enhanced after alkaline treatment, since recent reports indicate that alkaline treatment of quinoa saponins increase their biological activity. Six products were tested against B. cinerea: (1) non-purified quinoa extract, (2) purified quinoa extract, (3) alkali treated non-purified quinoa extract, (4) alkali treated purified quinoa extract, (5) non-purified quinoa extract treated with alkali but without thermal incubation and (6) purified quinoa extract treated with alkali but without thermal incubation. Untreated quinoa extracts showed minimum activity against mycelial growth of B. cinerea. Also, no effects were observed against conidial germination, even at 7mg saponins/ml. However, when the saponin extracts were treated with alkali, mycelial growth and conidial germination were significantly inhibited. At doses of 5mg saponins/ml, 100% of conidial germination inhibition was observed, even after 96h of incubation. Fungal membrane integrity experiments based on the uptake of the fluorogenic dye SYTOX green showed that alkali treated saponins generate membrane disruption, while non-treated saponins had no effects. The higher antifungal activity of alkaline treated saponins is probably due to the formation of more hydrophobic saponin derivatives that may have a higher affinity with the sterols present in cell membranes.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل National Agricultural Library