Impact of vte4 and crtRB1 genes on composition of vitamin-E and provitamin-A carotenoids during kernel-stages in sweet corn
2022
Chauhan, Hema S. | Chhabra, Rashmi | Rashmi, Tuhin | Muthusamy, Vignesh | Zunjare, Rajkumar U. | Mishra, Subhra J. | Gain, Nisrita | Mehta, Brijesh K. | Singh, Ashok K. | Gupta, Hari S. | Hossain, Firoz
Traditional sweet corn is poor in vitamin-E (tocopherols) and provitamin-A carotenoids (β-carotene and β-cryptoxanthin). Here, we analysed six biofortified sweet corn inbreds having mutant γ-tocopherol methyl transferase (vte4) and β-carotene hydroxylase1 (crtRB1) genes, and three traditional sweet corn inbreds with wild-type vte4⁺ and crtRB1⁺genes at 20-, 24- and 28-days after pollination (DAP). The biofortified inbreds possessed higher α-tocopherol (α-T: 2.03-folds), γ-tocopherol (γ-T: 1.19-folds), α-/γ-T (1.71-folds), β-carotene (BC: 6.21-folds), β-cryptoxanthin (BCX: 4.14-folds) and total provitamin-A (proA: 5.49-folds) over original inbreds. Vitamin-E attained peak at 24-DAP, while provitamin-A peaked at 20-DAP. Transcripts of vte4 and crtRB1 had the highest peak at 24-DAP. Biofortified inbreds recorded higher transcript level of vte4 (10-folds) and lower level of crtRB1 (8-folds) over vte4⁺ and crtRB1⁺. Vte4 transcript was positively associated with α-T, γ-T and α/γ-T. crtRB1 transcript had negative relationship with BC, BCX and proA. This is the first report on dynamic regulation of vte4 and crtRB1 genes during kernel-development among sweet corn.
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