Effects of nutrient supplementation with laminarin derived from Laminaria hyperborea and Laminaria digitata on mucin gene expression in the porcine ileum
2010
Ryan, M.T. | Smith, A.G. | O'Doherty, J.V. | Bahar, B. | Reilly, P. | Sweeney, T.
Mucins play an important role in protecting the mucosal epithelium from both enzymatic and microbial damage and act as a selective barrier for absorption in the small intestine. The aim of this study was to investigate the effects of nutrient supplementation with laminarin derived from the macroalgal species Laminara hyperborea and Laminara digitata on mucin gene expression in the porcine ileum. Total RNA was extracted from ileal tissues of euthanised pigs (n=7 per group); offered one of five dietary treatments: T1 basal (control) diet, T2 and T3 — basal diets with L. hyperborea at 300ppm or 600ppm, respectively, T4 and T5 — basal diets with L. digitata at 300ppm or 600ppm, respectively. Quantitative real-time PCR was used to quantify 7 mucin genes MUC1, 2, 4, 5AC, 12, 13 and 20. Five (MUC2, 4, 12, 13 and 20) of the 7 mucin genes examined were expressed in the porcine ileum. Dietary supplementation with extracts from L. hyperborea had no effect on any of the mucin genes investigated in this study. Dietary supplementation with extracts from L. digitata had a significant effect on only one mucin gene (MUC2; P=0.05). We conclude that nutrient supplementation with L. digitata derived laminarin had significant effects on expression of the predominant mucin (MUC2) in the porcine ileum.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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