Immobilization of lipase onto mesoporous magnetic nanoparticles for enzymatic synthesis of biodiesel
2016
Karimi, Mahmoud
Biodiesel production by enzymatic transesterification of waste cooking oil (WCO) to partially substitute petroleum diesel is considered as one of the measures for solving the twin problems of environment pollution and energy demand. High cost of lipases is considered as a main barrier to commercialize the enzymatic transesterification. In the present work, superparamagnetic iron oxide nanoparticles (SPION) have been synthesized, covered by Silica and then grafted by aldehyde groups in order to immobilize B. cepacia lipase, as a promising method enhancing the economic viability of enzymatic transesterification. The results demonstrated that the average size of SPIONs has been determined to be around 20nm. The nanoparticles linked to the lipases have displayed nearly superparamagnetic property. Effective variables on the enzymatic transesterification have been evaluated and the conversion of WCO to biodiesel reached 91% in methanol: oil molar ratio of 6:1, immobilized lipase concentration of 25wt%, n-hexane content of 10wt%, water content of 10wt%, reaction temperature of 35°C, and reaction time of 35h. Immobilized lipase could be recovered easily, used repeatedly, and the decrease in its activity was not largely observed.
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