Evaluation of genetic diversity in the world collection of Eruca sativa L. using oil content, fatty acids and molecular markers
2020
Golkar, Pooran | Bakhtiari, Mostafa Abdollahi
Arugula (Eruca sativa L.) an oilseed crop with various medicinal and industrial applications. In the present study, 66 genotypes of E. sativa were evaluated for genetic diversity based on the content of seed oil, nine different fatty acids, and molecular markers including 20 polymorphic sequence-related amplified polymorphisms (SRAP) and 12 polymorphic Start Codon Targeted (SCoT). Based on the results of analysis of variance, a significant difference was found among the genotypes in terms of oil quality-related traits. Among fatty acids, least content was denoted to eicosenoic acid (0–3.45 %), whereas the most content (25.9–53.6 %) was observed at erucic acid. The SRAP markers produced a higher mean number of polymorphic bands (8.1) and polymorphism (80.4 %) compared with SCoT markers (7.25 polymorphic bands with 80.35 % polymorphism. The higher genetic variation within populations revealed than among them for both evaluated markers. Thus, the combined SRAP and SCoT markers were significantly useful for genetic diversity evaluation at the molecular level in E. sativa. Furthermore, cluster analysis divided the genotypes into five groups based on a combined data of SRAP and SCoT, which was supported by structure analysis. Based on the results, no considerable similarity was observed between the geographic origin of genotypes with classification patterns based on molecular markers and oil quality-related traits. The results of the present study confirmed the effect of employing the mixture of SRAP and SCoT, regarding identifying E. sativa genetic diversity that would be useful for improving population genetics and superior hybrids in E. sativa in further breeding studies on this oily crop.
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