Detection of interstitial telomeric sequences in the Arctic charr (Salvelinus alpinus) (Teleostei: Salmonidae)
2011
Pomianowski, K. | Jankun, M. | Ocalewicz, K.
Highly polymorphic Arctic charr (Salvelinus alpinus Linnaeus, 1758) chromosomes were studied using conventional and molecular methods. The diploid chromosome number in the studied individuals was 2n = 81 or 2n = 82, with a fundamental arm number (NF) = 100. These differences are due to Robertsonian fusions. Interindividual variation in the number and size of DAPI and CMA₃ positively stained chromatin sites was observed in studied specimens. In the case of two individuals, the subtelomeric region of the long arm (q) of the largest acrocentric chromosome (chromosome number 10) was positively stained by CMA₃ fluorochrome. Both primed in situ labelling (PRINS) and fluorescence in situ hybridization (FISH) revealed that this CMA₃-positive region was flanked by telomeric sequences. Previously, the subterminal position of interstitial telomeric sequences located in the vicinity of the CMA₃-positive guanine-rich chromatin have been described in two other Salvelinus species, brook trout (Salvelinus fontinalis) and lake trout (Salvelinus namaycush). Moreover, multichromosomal location and variation in size of CMA₃ bands have been observed in various Salvelinus taxa, including fishes with internally located telomeric sequences. These results suggest that relocation of CMA₃-positive chromatin segments in these species may be facilitated by flanking interstitial telomeric sequences (ITSs).
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