Survey of antibiotics residues in DDGS from 14 different states by LCMS
2021
Olendorff, Samantha A. | Prasad, Karolina | Tucker, Kevin R.
BACKGROUND AND OBJECTIVES: Antibiotics are used in the corn‐based ethanol production to control the proliferation of bacteria during fermentation of starch to ethanol. Dried distiller's grains with solubles (DDGS) is a by‐product of corn‐based fermentation that can effectively be used as feed for cattle, swine, chicken, and other livestock. High amounts of antibiotic residues in the feed may lead to antibiotic resistant bacteria. FINDINGS: Antibiotics were extracted from DDGS samples by liquid‐liquid extraction, purified by solid phase extraction, and quantified by liquid chromatography mass spectrometry in multiple reaction monitoring mode to quantify five antibiotics: penicillin G, erythromycin, tylosin, virginiamycin M1, and virginiamycin S1. DDGS was collected and analyzed quarterly over the course of ten months to obtain a representation of DDGS produced in various parts of the United States in different seasons. CONCLUSIONS: Penicillin G was detected in a single sample. Tylosin was detected in 28%, erythromycin in 45%, virginiamycin M1 in 53%, and virginiamycin S1 in 83% of the total samples. SIGNIFICANCE AND NOVELTY: Largest study to date to quantify antibiotics in DDGS (33 ethanol plants in 14 states).
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