Isolation and characterisation of <i>Mycobacterium avium</i> and <i>Rhodococcus equi</i> from granulomatous lesions of swine lymph nodes in Slovenia
2004
Pate, Mateja | Zdovc, Irena | Pirš, Tina | Krt, B. | Ocepek, M.
Granulomatous lesions in bovine and especially swine lymph nodes are still frequently observed during routine veterinary meat inspections even though <i>Mycobacterium bovis</i> infections are no longer detected in domestic animals in Slovenia. Different lymph nodes of pigs (n = 260) were investigated using classical bacteriological and molecular methods. <i>Mycobacterium avium</i> alone was isolated in 47.3% of pigs and in mixed infection with <i>Rhodococcus equi</i> in 3.9% of pigs. <i>R. equi</i> alone was isolated in 27.3% and in mixed infection with mycobacteria other than <i>M. avium</i> in 1.5% of pigs. A total of 133 <i>M. avium</i> isolates were typed using the IS<i>1245</i>, IS<i>901</i> and FR<i>300</i> PCR. Almost two thirds (60.9%) of isolates belonged to <i>M. avium hominissuis</i> (IS<i>901</i>-, IS<i>1245</i>+ genotype), 33.8% of isolates belonged to <i>M. avium avium</i> (IS<i>901</i>+, IS<i>1245</i>+ genotype) and 5.3% of isolates remained non-typed. Fifty out of 85 <i>R. equi</i> isolates were tested for the virulence-associated antigens (VapA and VapB). Nearly two thirds (60.0%) were positive for VapB while all the other isolates were VapA- and VapB-negative.
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