The Ag⁺–G interaction inhibits the electrocatalytic oxidation of guanine – A novel mechanism for Ag⁺ detection
2011
Liu, Xingxing | Li, Wang | Shen, Qinpeng | Nie, Zhou | Guo, Manli | Han, Yitao | Liu, Wei | Yao, Shouzhuo
The heavy metal ions–nucleobases interaction is an important research topic in environmental and biochemical analysis. The presence of the silver ion (Ag⁺) may influence the formation of oxidation intermediate and the electrocatalytic oxidation activity of guanine (G), since Ag⁺ can interact with guanine at the binding sites which are involved in the electrocatalytic oxidation reaction of guanine. According to this principle, a new electrochemical sensor for indirectly detecting Ag⁺ based on the interaction of Ag⁺ with isolated guanine base using differential pulse voltammetry (DPV) was constructed. Among the heavy metal ions examined, only Ag⁺ showed the strongest inhibitory effect on the electrocatalytic oxidation of guanine at the multi-walled carbon nanotubes modified glassy carbon electrode (CNTs/GC). And the quantitative study of Ag⁺ based on Ag⁺–G sensing system gave a linear range from 100nM to 2.5μM with a detection limit of 30nM. In addition, this modified electrode had very good reproducibility and stability. The developed electrochemical method is an ideal tool for Ag⁺ detection with some merits including remarkable simplicity, low-cost, and no requirement for probe preparation.
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