A novel reaction-based fluorescent probe for the detection of cysteine in milk and water samples
2018
Wang, Jialin | Wang, Hao | Hao, Yanfeng | Yang, Shaoxiang | Tian, Hongyu | Sun, Baoguo | Liu, Yongguo
A novel fluorescent probe 3′-hydroxy-3-oxo-3H-spiro [isobenzofuran-1,9′-xanthene]-6′-yl-2,4-dinitrobenzenesulfonate (probe 1) was designed and synthesized as a visual sensor for the detection of cysteine levels in milk and water samples. The addition of cysteine to the solution of probe 1 resulted in an increase in fluorescence intensity and color change, from light yellow to yellow-green. The distinct color response indicated that probe 1 could be used as a visual sensor for cysteine. Cysteine can be detected quantitatively at concentrations between 0 and 400 μM and the detection limit of the fluorescence response to the probe was 6.5 μM. This suggests that probe 1 could be used as a signaling tool to determine the cysteine levels in samples, such as milk and water.
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