'Normalization' of germfree mice with anaerobically cultured caecal flora of 'normal' mice
1984
Koopman, J. P. | Kennis, Henriëtte M. | Mullink, J. W. M. A. | Prins, R. A. | Stadhouders, A. M. | Boer, H. de | Hectors, Magda P.
Germfree (GF) mice were inoculated with a cultured flora from 10⁻¹, 10⁻³, 10⁻⁵, and 10⁻⁷ dilutions of caecal contents from a 'normal' mouse. GF mice associated with a flora of a 'normal' mouse served as controls. The following intestinal parameters were determined: Colonization resistance (CR), Relative caecal weight (RCW), villus:crypt ratio (jejunum and ileum), IgA-producing cells (jejunum and ileum), ß-aspartyl glycine (faeces), volatile and non-volatile fatty acids (caecum) and bile acids (faeces). Only the 10⁻¹ culture was able to induce similar changes in the GF mice to a 'normal' flora. The GF + 10⁻⁵ and GF + 10⁻⁷ groups deviated markedly from the controls while the GF + 10⁻³ group showed in general intermediate values between GF + SPF and GF + 10⁻¹ on the one hand and GF + 10⁻⁵ and GF + 10⁻⁷ on the other hand. ß-aspartyl glycine was present only in the GF + 10⁻⁷ group. Scanning electron microscopy (SEM) of ileal contents revealed segmented filamentous organisms in the ileum of controls and the GF + 10⁻¹ group. The faecal flora consisted mainly of fusiform organisms. In the faeces of the 10⁻⁵ and 10⁻⁷ groups increasing amounts of non-bacterial matter were found, while in the faeces of the other groups virtually only bacteria were seen.
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