Biochemical CuSO4 Toxicity in Drosophila melanogaster Depends on Sex and Developmental Stage of Exposure
2019
Halmenschelager, Paula Tais | da Rocha, João Batista Teixeira
Copper is a transition metal that exists in different chemical forms (e.g., Cu²⁺,Cu⁺, and Cu⁰) and at high concentrations it is toxic. Here, we investigated the Cu²⁺-induced toxicity in Drosophila melanogaster, evaluating the survival, locomotion, and the activity of acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes. Flies were exposed to Cu²⁺(0.1–1 mmol CuSO₄/kg of diet or approximately 0.1–1 mM Cu²⁺) and allowed to mate during 24 h. GST and AChE enzymes were evaluated in the larvae and in the head and the body (thorax + abdomen) of the adult male and females flies. The total number of adult females (0.4–1 mM) and males (0.75 and 1 mM) was decreased by CuSO₄. The climbing ability was hampered in flies exposed to 1 mM Cu²⁺. In larvae, Cu²⁺(0.4–1 mM) increased AChE activity (P < 0.002). In males’ heads, 0.4 mM Cu²⁺ increased the AChE activity (P < 0.01). In adults’ bodies, Cu²⁺inhibited the activity in both sexes, but with greater effectiveness in males (0.1 to 1 mM) than in females (1 mM). Regarding GST activity, 0.1 mM Cu²⁺increased, but 1 mM decrease GST in larvae. In the head of flies, Cu²⁺decreased the GST activity at intermediate (0.4 mM) and increased GST at the highest concentration (1 mM) in males. In the bodies, the effect of Cu²⁺was similar. In conclusion, Cu²⁺exposure in D. melanogaster disrupted locomotion and enzymatic parameters that can be related to changes in AChE and in the detoxifying GST enzyme.
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