Tissue specific-distribution and metabolism of vitamin A are affected by dietary protein levels in rats
1998
Furusho, T. | Wada, M. | Yasuhara, T. | Kataoka, E. | Kato, S. | Masushige, S.
The effect of dietary protein levels on tissue-specific distribution and metabolism of vitamin A was studied in rats given [15-(14)C] retinol (14C-ROH) The weanling rats were fed a low level vitamin A diet for 10 days, then rats (15 rats per group) were divided into 2 groups; one was given a 40% casein diet as a high protein diet (HP-diet), and the other a 5% casein diet as a low protein diet (LP-diet). After 10 days feeding on these diets, 14C-ROH (5 microcurie/rat) was given to both groups. HP-diet and LP-diet, by intraperitoneal injection. The radioactivity in the exhalated gases, urine and feces was measured to estimate the rate of vitamin A metabolism. The tissue specific-distribution of ROH was studied in terms of the radioactivities of the ROH fractions separated by HPLC. The hepatic 14C-ROH content in the HP-diet group was lower than that in the LP-diet group at 24, 48, and 72 hours after administration of 14C-ROH. In contrast, 14C-ROH content in serum. spleen, pancreas. and small intestinal mucosa in the HP-diet group was higher than that in the LP-diet group. The radioactivity of the exhalated gas and feces was higher in the HP-diet group. These results suggest that metabolism of vitamin A is higher with intake of a HP-diet. Thus, dietary protein levels may affect tissue-specific distribution and metabolism of vitamin A, there-by modulating the actions of this vitamin.
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