Induction of aldose reductase activity in Candida guilliermondii by pentose sugars
1995
Sugai, J.K. | Delgens, J.P.
Cell extracts of Candida guilliermondii grown in D-xylose, L-arabinose, D-galactose, D-glucose, D-mannose and glycerol as sole carbon sources possessed NADPH-dependent aldose reductase activity, but no NADH-dependent activity was detected. D-xylose and L-arabinose were the best inducers of aldose reductase activity. The highest enzyme activity in D-xylose or L-arabinose-grown cells was observed first with L-arabinose followed by D-xylose as substrates of the enzymatic reaction. However, only low activity was found in D-glucose, D-mannose and D-galactose-grown cells, indicating that these carbon sources cause catabolite repression. Enzyme activities induced in D-xylose-grown cells were twice as high as those obtained from the cells under resting conditions. Furthermore, the level of induction of aldose reductase activity depended on the initial concentration of D-xylose. The present study shows that aldose reductase activity may be efficiently induced by pentose sugars of hemicellulosic hydrolysates and weakly by hemicellulosic hexoses.
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