Methods for improving Xanthomonas vasicola pv. vasculorum infection in corn
2022
Hendges, Camila | Stangarlin, José Renato | Kuhn, Odair José | Tartaro, Eloisa Lorenzetti
Corn (Zea mays L). is widely cultivated and has great economic importance, but diseases are among the factors that affect production. In research, pathogen inoculation methods and the inoculum concentration are important factors when studying the infection process. The present study aimed to evaluate the efficiency of inoculation methods of the bacterium Xanthomonas vasicola pv. vasculorum (Xvv) and also its concentration to inform future work on the control of bacterial leaf streak in corn. In the first experiment, the methods of inoculation were studied by leaf spraying on hydathodes, stalk wounds, leaf wounds, or deposition of the inoculum within the whorl. For the inoculum quantification experiment, concentrations of 0, 10⁶, 10⁷, 10⁸, and 10⁹ CFU (colony-forming unit) mL⁻¹ were used. Inoculations were carried out at phenological stage V4 on plants kept in a greenhouse, the severity was evaluated, and the area under the disease progress curve (AUDPC) was calculated. There were no symptoms of inoculation via hydathodes. The methods of inoculation by leaf spraying and inoculum deposition within the whorl provided the highest intensities of disease, followed by the stalk wounds inoculation method. Regarding the inoculum concentration, the highest AUDPC was found in 10⁸ CFU mL⁻¹. Thus, these methods and the determined optimal inoculum concentration provide useful information for further assays aiming at the control of bacterial leaf streaks in corn.
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