Bacterial communities of Beijing surface waters as revealed by 454 pyrosequencing of the 16S rRNA gene

Wei, Yu-Mei; Wang, Jing-Qi; Liu, Ting-Ting; Kong, Wei-Wen; Chen, Nan; He, Xiao-Qing; Jin, Yi

Bacterial communities of Beijing surface waters as revealed by 454 pyrosequencing of the 16S rRNA gene

2015

As a better understanding of Beijing surface water ecosystems can provide clues for environmental management and public health, here, we report a study of the bacterial communities of five Beijing surface waters conducted using 454 pyrosequencing of 16S ribosomal RNA (rRNA) genes. We expected to observe a core bacterial community among the surface waters and differences in bacterial community abundance over the different locations of sampling. In this study, we obtained a total of 60,810 trimmed reads from the five samples after the removal of unqualified reads. Bacterial sequences from the five samples were classified into taxonomic classes using the default settings of the mothur platform. Our results provided insight into the bacterial community composition of surface waters and revealed that there was a core microbial community in the microbial populations of surface samples at different geographic locations, with 13 phyla and 40 genera in common. Our findings also revealed the differences in bacterial communities among five surface water samples obtained at different locations.

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