Chrysolaminarin biosynthesis in the diatom is enhanced by overexpression of 1,6-β-transglycosylase
2022
Yang, Yu-Feng | Li, Da-Wei | Balamurugan, Srinivasan | Wang, Xiang | Yang, Wei-Dong | Li, Hong-Ye
Chrysolaminarin is the major form of storage polysaccharides in the diatom Phaeodactylum tricornutum, which is mainly composed of β-1,3-glucan with β-1,6-branched chains (molecular weight at 10 kDa). Chrysolaminain has various biological activities such as antioxidant, anti-tumor and immune enhancement. 1,6-β-transglycosylase is speculated to be the last step in the synthesis of chrysolaminain by adding β-1,6-branched chains to the main chain of β-1,3-glucan. In this study, the putative 1,6-β-transglycosylases (PtTGS1) in P. tricornutum was characterized. PtTGS1 was overexpressed and significantly promoted the chrysolaminain synthesis and reduced lipid synthesis. The continuous accumulation of chrysolaminain was achieved by influencing the shunt of carbon metabolism without affecting the normal physiological state (growth and photosynthesis) of algal cells. These results indicated the important role of PtTGS1 gene in chrysolaminain synthesis and carbon shunt pathways.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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