Long-term culture of the marine dinoflagellate microalga Amphidinium carterae in an indoor LED-lighted raceway photobioreactor: Production of carotenoids and fatty acids
2018
Molina-Miras, A. | López-Rosales, L. | Sánchez-Mirón, A. | Cerón-García, M.C. | Seoane-Parra, S. | García-Camacho, F. | Molina-Grima, E.
The feasibility of the long-term (>170 days) culture of a dinoflagellate microalga in a raceway photobioreactor is demonstrated for the first time. Amphidinium carterae was chosen for this study as it is producer of interesting high-value compounds. Repeated semicontinuous culture provided to be a robust operational mode. Different concentration levels of the f/2 medium nutrients (i.e. f/2×1–3) were assayed. The composition f/2×3 (N:P = 5), combined with a sinusoidal irradiance pattern (L/D = 24:0) with a 570 µE m⁻² s⁻¹ daily mean irradiance, maximized the biomass productivity (2.5 g m⁻² day⁻¹) and production rate of the valuable carotenoid peridinin (19.4 ± 1.35 mg m⁻² L⁻¹ with nearly 1% of the biomass d.w.). Several carotenoids and polyunsaturated fatty acids were also present in significant percentages in the harvested biomass (EPA, 1.69 ± 0.31% d.w.; DHA, 3.47 ± 0.24% d.w.), which had an average P-molar formulate of C₄₀.₇O₂₁.₂H₇₃.₉N₃.₉S₀.₃P₁.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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