Identification of molecular markers for the detection of the yellow rust resistance gene Yr17 in wheat
1999
Robert, O. | Abelard, C. | Dedryver, F.
The Yr17 gene, which is present in many European wheat cultivars, displays yellow rust resistance at the seedling stage. The gene introduced into chromosome 2A from Aegilops ventricosa was previously found to be closely linked (0.5 cM) to leaf and stem rust resistance genes Lr37 and Sr38, respectively. The objective of this study was to identify molecular markers linked to the Yr17 gene. We screened with RAPD primers, for polymorphism, the DNAs of cs. Thatcher and the leaf rust-resistant near-isogenic line (NIL) RL 6081 of cv. Thatcher carrying the Lr37 gene. Using a F2 progeny of the cross between VPM1 (resistant) and Thesee (susceptible) the RAPD marker OP-Y15(580) was found to be closely linked to the Yr17 gene. We converted the OP-Y 15(580)RAPD marker into a sequence characterized amplified region SCAR). This SCAR marker(SC-Y15) was linked at 0.8+/-0.7 cM to the Yr17 resistance gene. We tested the SC-Y15 marker over a survey of 37 wheat cultivars in order to verify its consistency in different genetic backgrounds and to explain the resistance of some cultivars against yellow rust. Moreover, we showed that the Xpsr150-2Mv locus marker of Lr gene described by Bonhomme et al.[6] which possesses A. ventricosa introgression on the 2A chromosome was also closely linked to the Yr17 gene. Both the SCAR SC-Y15 and Xpsr150-2M markers should be used in breeding programmes in order to detect the cluster of the three genes yr17, Lr37 and Sr38 in cross progenies.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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