Quantitative and qualitative analysis of retinoids in Artemia and copepods by HPLC and diode array detection
2005
Moren, M. | Gundersen, T.E. | Hamre, K.
Different concentrations of retinoids have been reported in Artemia. The levels vary from 0-362 microgram g(-1) Artemia (dry wt.). The aim of this study was to investigate the actual retinoid concentration in Artemia and compare it with levels in copepods. Samples of both DC-DHA Selco (INVE, Ghent, Belgium) from the bottle and after 12 h in the enrichment tank in addition to enriched and unenriched Artemia and copepods from a pond system were collected and analysed for vitamin A components. The analyses were conducted using two different HPLC (high pressure liquid chromatography) methods to ensure the justness of the results. The DC-DHA Selco contained 132.7 microgram vitamin A g(-1). After 12 h in the enrichment tank we could not detect any vitamin A. None of the following retinoids were observed in Artemia or copepods with any of the methods: all-trans-retinol (at-ROH), 9-cis-ROH or at-3,4-didehydro-ROH (at-3,4-dd-ROH). Method 2 detects several other retinoids when present, but none of the following were found in the two live prey organisms: cis or trans 3,4-dd-RA, cis or trans 3,4-dd-ROH, cis or trans 3,4-dd-RAL, cis or trans ROH or cis or trans RA. This implies that larvae feeding on either copepods or Artemia must meet their retinoid requirement from other sources than retinoids.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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