Gene expression and plasma lipid content in relation to intramuscular fat in Chinese indigenous Wuhua chicken
2017
Chen, Xingyong | Niu, Juanjuan | Geng, Zhaoyu
The deposition pattern of intramuscular fat (IMF), inosine monophosphate (IMP), and fat metabolism related gene expression profiles were studied in Wuhua chickens. A total of 160 healthy birds (80 of each gender) was reared. Ten birds from each gender were randomly selected at 90, 120, 150, and 180 d of age for blood and tissue sample collection. There were no changes in male IMF content that ranged from 0.9 to 1.79 mg/g. In females, there were large differences in IMF content, which increased from 0.62 mg/g at 90 d to the highest level of 2.61 mg/g at 150 d (P = 0.015). No statistical changes were observed in IMP content in both males and females. In females, very low density lipoprotein (VLDL) exhibited an age dependent manner and reached the highest level of 64.93 mg/mL at 180 d, while the low density lipoprotein (LDL) reached the highest level at 120 d (1.35 nmol/L). Serum triglycerides (TG) increased to the highest level of 1.3 nmol/L at 180 d (P = 0.0003). In males, TG reached the highest level at 120 d (P = 0.035). The A-FABP mRNA expression was highest at 180 d of age in males (P = 0.017). The Apo-B and PPAR-γ genes expressed the highest levels around 150 d of age in female chickens. Higher plasma TG was associated with greater breast IMF in both males and females. The correlation coefficient between IMF and PPAR-γ expression was 0.875 (P = 0.065) in females. Plasma TG might be used as a potential index for breast IMF selection, and PPAR-γ might be used as a candidate gene for IMF selection in females.
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