Removal of genotoxicity in chlorinated secondary effluent of a domestic wastewater treatment plant during dechlorination

PURPOSE: Dechlorination with tetravalent sulfur is widely used in wastewater treatment processes after chlorination. Dechlorination can remove certain genotoxic disinfection by-products (DBPs). However, the reactions occurring during dechlorination of chlorinated secondary effluent and their genotoxic chemicals are still very complex, and the related genotoxicity changes remain unknown. Therefore, the effects of dechlorination on genotoxicity in secondary effluent and its fractions and typical genotoxic chemical after chlorination were evaluated. METHODS: The dissolved organic matter in the secondary effluent sample was separated into four fractions with XAD-8 resin. Genotoxicity of secondary effluent and its fractions was evaluated by SOS/umu test, an ISO standard method. The concentration of typical genotoxic chemical named ofloxacin was determined by liquid chromatography with a mass spectrometer and a fluorescence detector. RESULTS: Dechlorination with the addition of Na2SO3 notably decreased the genotoxicity in the chlorinated secondary effluent, especially in the presence of high ammonia nitrogen concentration in the sample before chlorination. The Na2SO3 addition significantly decreased the genotoxicity of the secondary effluent and its genotoxic ofloxacin prior to chlorination. The genotoxicity in the fractions containing hydrophobic acids (HOA) increased after chlorination, while addition of Na2SO3 decreased the genotoxicity induced by chlorination. Tryptophan found in HOA exhibited genotoxicity after chlorination, while dechlorination decreased the genotoxicity in chlorinated tryptophan induced by DBPs. CONCLUSIONS: Dechlorination was found to decrease the genotoxicity of chlorinated secondary effluent. The decrease was associated with the reduction of genotoxicity in genotoxic chemicals in secondary effluent prior to chlorination and DBPs.