The effect of light quality on leaf production and development of in vitro-cultured plants of Alternanthera brasiliana Kuntze

Macedo, Andrea Furtado; Leal-Costa, Marcos Vinícius; Tavares, Eliana Schwartz; Lage, Celso Luiz Salgueiro; Esquibel, Maria Apparecida

The effect of light quality on leaf production and development of in vitro-cultured plants of Alternanthera brasiliana Kuntze

2011

Macedo, Andrea Furtado | Leal-Costa, Marcos Vinícius | Tavares, Eliana Schwartz | Lage, Celso Luiz Salgueiro | Esquibel, Maria Apparecida

We investigated the influence of light quality on the leaf development of Alternanthera brasiliana Kuntze (Amaranthaceae) grown in vitro. Growth parameters including specific leaf mass, thickness, and leaf density were lowest in plants grown under red light. Blue light induced the largest number of leaves/plant, and the largest thickness and area of the leafblade. Green and red lights induced the smallest leaf areas. The thickness of the abaxial-face epidermis and spongy parenchyma of the plants was significantly reduced in plants grown under red light. The thickness of the palisade parenchyma and upper epidermis were significantly increased in plants grown under blue light, compared to the other fluorescent-light treatments. The specific spectral band also influenced the differentiation of mesophyll cells. In the dark and under red light, the mesophyll was homogenous; and in the dark and under green light, the leaves were more compact. Under blue light, the cells displayed the characteristic palisade morphology. The results showed that the increase of a specific parenchyma type was related to a specific spectral band. All spectral-quality treatments reduced the numbers of stomata and trichomes. The results for green light were in some respects similar to those for red light, and in other respects similar to those for blue light, probably because phytochromes and cryptochromes are green-light receptors. This study indicated that Alternanthera plants have strong morphological plasticity induced by light. The results suggest that high-quality Alternanthera can be achieved by culturing the plants in vitro under a combination of blue and red light.

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