In this research, 42 mesir paste samples produced by a limited number of manufacturers in Manisa, Turkey, were analysed for aflatoxin content by high-performance liquid chromatography with a fluorescence detector after pre-separation using immunoaffinity columns. A good correlation was found with good performance in terms of precision for the method. Limit of detection-limit of quantitation of AFG ₂-AFB ₂ and AFG ₁-AFB ₁ were 0.05/0.15 and 0.03/0.09 µg/kg, respectively. AFG ₂, AFG1 and AFB ₁ were detected in 29%, 71% and 52% samples, whereas AFB ₂ was not detected in any sample. AF ₜₒₜₐₗ, AFG ₂, AFG ₁ and AFB ₁ content varied between 0.04/10.20 µg/kg, 0.07/0.42 µg/kg, 2.02/10.11 µg/kg and 0.04/0.11 µg/kg, respectively. About 9.5% of samples were found to be above the maxium limit (ML) for AF ₜₒₜₐₗ (10 µg/kg), but none of samples exceeded the ML for AFB ₁ (5 µg/kg) as set by the European Union for spices. According to the results, it was concluded that traditional products like mesir paste which contains different types of spices should be examined in terms of aflatoxins.
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