New tools for breeding cantaloupe melons: first introgression line collection of makuwa melons into Charentais genetic background
2017
Perpina, G. | Esteras, C. | Monforte, A. J. | Pico, M. B.
A large number of single nucleotide polymorphisms (SNP) have been developed recently in melon, representing a resource that facilitates the development of new breeding populations. Introgression lines (IL) collections are suitable to the study of quantitative traits and, especially, to introduce new exotic genetic variability into modern cultivars. We have developed a new IL collection, employing the Japanese cultivar 'Ginsen makuwa', subsp. agrestis var. makuwa as exotic donor parent and the French Charentais-type cultivar 'Vedrantais', subsp. melo var. cantalupensis as the recurrent parent. Successive backcrosses and selfing generations (BC2, BC3, BC3S1, BC3S2 and BC4S1) were genotyped with SNP uniformly distributed throughout the melon genome by Sequenom MassARRAY and High Resolution Melting assays. In each generation, plants with higher recurrent genome, but representing the whole makuwa genome in the whole population, were selected. The final IL population covered nearly 100% of the donor genome. Fruit quality traits were assessed and quantitative trait loci (QTL) could be mapped for some of these traits. Some QTL involved in sugar content, fruit shape, flesh firmness and flesh color showed consistent effects across generations. Some of these QTL coincide with previously reported QTL, but some of them map in genomic regions where QTL were not reported before. Remarkably, some makuwa IL were associated with an increase in fruit sugar content. Some IL displayed combined phenotypes interesting for cantaloupe breeding, such as delayed climacteric associated with a higher sugar content.
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