Improved tolerance to bile salts of aggregated Bifidobacterium longum produced during continuous culture with immobilized cells
2011
Reimann, Sebastian | Grattepanche, Franck | Benz, Rafael | Mozzetti, Valeria | Rezzonico, Enea | Berger, Bernard | Lacroix, Christophe
The effect of cell immobilization and continuous culture was studied on selected physiological and technological characteristics of Bifidobacterium longum NCC2705 cultivated for 20days in a two stage continuous fermentation system. Continuous immobilized cell (IC) cultures with and without glucose limitation exhibited formation of macroscopic cell aggregates after 12 and 9days, respectively. Auto-aggregation resulted in underestimation of viable cell counts by plate counts by more than 2log units CFU/ml compared with qPCR method. Modifications of cell membrane composition might partially explain aggregate formation in IC cultures. Decreases in the ratio of unsaturated to saturated fatty acid content from 1.74 to 0.58 might also contribute to the enhanced tolerance of IC cells to porcine bile salts and aminoglycosidic antibiotics compared with free cells from batch cultures. The enhanced resistance against bile salts in combination with auto-aggregation may confer an advantage to probiotic bacteria produced by IC technology.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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