A novel fluorescent probe with a large Stokes shift for real-time imaging mitochondria in different living cell lines
2017
Meng, Fangfang | Liu, Yong | Niu, Jie | Lin, Weiying
Fluorescent dyes with large Stokes shift play a key role in avoiding self-quenching and scattered light of dyes in the process of biological imaging. In this work, a novel mitochondria-targetable fluorescent dye (PI-C2) with large Stokes shift (e. g. Maxium value is 219 nm in DMSO) have been developed. Compared to the commercial mitochondria probes MTR and MTG (Less than30nm in various solution), the newly constructed PI-C2 has a much larger Stokes shift in various solutions (169-219 nm in various solutions). Furthermore, the probe can successfully be applied for sensing mitochondria, and exhibited excellent photostability in different living cell lines. The novel fluorescent platform with the large Stokes may be extended to construct powerful fluorescent probes with large Stokes shift for detecting a wide variety of biomolecules in mitochondria.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل National Agricultural Library