Effects of terbinafine on growth, squalene, and steryl ester content of a celery cell suspension culture
1991
Yates, P.J. | Haughan, P.A. | Lenton, J.R. | Goad, L.J.
The allylamine terbinafine inhibited growth of a celery (Apium graveolens) cell suspension culture (I50 = 90 micromoles) and blocked the action of squalene epoxidase, resulting in an accumulation of squalene and a decrease in the sterol content of the cell. Celery cells were tolerant to squalene accumulation; inhibition of growth of cultures was associated with a fall in the free sterol content below about 1 microgram sterol/mg dry weight of cells. At Day 14, untreated celery cells contained about 60% of the total sterol in the esterified form. However, the steryl ester pool was considerably lower in terbinafine-treated cells which may reflect an attempt to maintain the free sterol content above a threshold value. The composition of free sterols of terbinafine-treated cells was different from control cultures, suggesting that terbinafine has a second site of inhibition in the pathway to major sterols in plant.
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