In-silico and in-vitro evaluation of the potential of maize kernels to inhibit trypsin activity
2015
Brugger, D. | Loibl, P. | Schedle, K. | Windisch, W.M. | Fahn, C.
A study has been conducted in order to evaluate the potential of maize kernels to inhibit trypsin activity in-silico and in-vitro. Assessment of published proteomic data revealed at least one specific trypsin inhibiting peptide within the Zea mays proteome with significant homology to proteins in other grain species but not legumes. Subsequently, a total of 12 maize kernel samples from two countries (Germany and Austria) were screened for their native trypsin inhibitor activity (TIA). The average TIA was 1.27±0.33mg/g dry matter (DM), ranging between 0.56 and 1.87mg/g DM, and was no longer detectable after autoclaving (125°C, 250kPa, 1h). Moreover, the degree of inhibition seemed to correlate positively with the crude protein content (r=+0.68*). The trypsin inhibiting effect declined through stepwise addition of exogenous trypsin, highlighting its specific suicidal mode of action. The optimal temperature for denaturation of the inhibitor pool was 82±1°C. Our results provide, to our knowledge for the first time, information about the trypsin inhibiting potential of maize kernels in-vitro. Furthermore, we identified several significant homologies between a maize bound trypsin inhibitor and peptides which are abundant in the proteomes of other grain species.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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