A facile method for detection of alkaline phosphatase activity based on the turn-on fluorescence of resorufin
2014
Wang, Fangyuan | Li, Yongxin | Li, Wenying | Zhang, Qingfeng | Chen, Jian | Zhou, Huipeng | Yu, Cong
In the present study, a facile fluorescence turn-on approach for the detection of alkaline phosphatase (ALP) activity has been developed. l-ascorbic acid-2-phosphate (AAP), a substrate of ALP, could be hydrolyzed by ALP to give l-ascorbic acid. l-ascorbic acid then reduced resazurin to resorufin, which resulted in a turn-on fluorescence signal. The fluorescence intensity increase could be directly related to the amount of ALP added to the assay solution. The assay is very sensitive, as 0.12 mU mL⁻¹ ALP could be clearly detected. Sodium orthovanadate (Na₃VO₄), a well-known ALP inhibitor, was tested, and a clear inhibition effect was observed. The results suggest that our method could be used for ALP activity sensing related various biochemical applications and for the screening of ALP inhibitors.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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