Impact of slicing hygiene upon shelf life and distribution of spoilage bacteria in vacuum packaged cured meats

A model for studying spoilage in sliced cooked delicatessen meats using conventional plating procedures was characterized by following the transfer of adventitious lactic acid bacteria (LAB), thermotolerant LAB and pediococci from a dry fermented sausage to cooked ham and bologna during a simultaneous slicing procedure. The major group of organisms present in these products was LAB. Each of the three groups of organisms was transferred to the cooked meats, but during storage under vacuum at 7 degrees C, only the adventitious lactobacilli from the sausage appeared to contribute to cooked meat spoilage. Pediococci declined within 28 days of co-slicing, thermotolerant LAB grew slowly and never reached levels of > 5 log10 cfu cm-2, but transferred adventitious LAB from the sausage grew and reached peak populations 4 weeks earlier in the co-sliced cooked meats than in controls. During storage at 7 degrees C co-sliced cooked meats spoiled, as assessed by purge (liquid) and ropy slime formation, 2-4 weeks faster than the separately-sliced and vacuum-packaged products. The distribution of organisms on package slices differed. In control cooked products numbers of bacteria present on center package slices were lower than on outside slices. Bacterial numbers were greatest at the meat surface-packaging film interface. In co-sliced cooked products organisms were evenly distributed throughout slices in the packages. Based on findings from use of the co-slice model, in vacuum packages of sliced cured meats where > 10(5) cfu bacteria cm-2 are equally distributed throughout package slices, poor slicing hygiene is the cause. Commercially-sliced bologna was repackaged with allyl isothiocyanate (AIT) to test its ability to delay bacterial growth at the meat surface. The preservative effects of AIT were tested under vacuum or when backflushed with CO2. CO2 alone was more effective than AIT in delaying LAB growth but neither treatment was particularly effective.